Models, laws and the limits of reductionism

I am currently reading Stuart Kauffman's "Reinventing the Sacred" and it's turning out to be one of the most thought-provoking books I have read in a long time, full of mind-bending ideas. Kauffman who was originally trained as a doctor was for many years a member of the famous Institute for Complexity in Santa Fe, which is a bastion of interdisciplinary research.

Kauffman is a kind of polymath who draws upon physics, chemistry, biology, computer science and economics to essentially argue the limitations of reductionism and the existence of emergent phenomena. He makes some fascinating arguments for instance about biology not being reducible to specific physics. One of the main reasons this cannot be done is because the evolution of complex biological systems is contingent and can follow any number of virtually infinite courses depending on slightly different conditions; according to Kauffman, this infinity is not just a ‘countable infinity’ but an ‘uncountable one’ (more on this mind-boggling distinction later). Biological systems are also highly non-linear and full of feedback and 'surprises' and these qualities make their prediction not just very difficult in practice but even in principle.

I am sure I will have much more to say about Kauffman’s book later, but for now I want to focus on his argument against reductionism based on what is called the ‘multiple platform’ framework. Kauffman’s basic thesis draws on an argument made by the Nobel laureate Philip Anderson. Anderson wrote a groundbreaking article in Science in 1972 extolling the limits of reductionism. To illustrate the multiple platform principle, he talked about computers processing 1s and 0s and manipulating them to give a myriad number of results. The question is: is the processing of 1s and 0s in a computer uniquely dependent upon the specific physics involved (which in this case would be quantum mechanics)? The answer may seem obvious but Anderson says that it’s hard to make this argument, since one can also get the same results from manipulating buckets that are either empty (0s) or filled with water (1s). Thus, the binary operations of a computer cannot be reduced to specific physics since they can be modeled by ‘multiple platforms’.

Another example that Kauffman cites is of the Navier-Stokes equations, the basic equations of fluid dynamics. The equations are classical and are derived from Newton’s laws. One would think that they would be ultimately reducible to the movements of individual particles of fluid and thus to quantum mechanics. Yet as of today, nobody has found a way to derive the Navier-Stokes equations from those of quantum mechanics. However, the physicist Leo Kadanoff has actually ‘derived’ these equations by using a rather simple ‘toy world’ of beads on a lattice. The movement of fluids and therefore the equations can be modeled by moving the beads around. Thus, we again have an example of multiple platforms leading to the same phenomenon, precluding the unique dependence of the phenomenon on a particular set of laws.

All this is extremely interesting, but I am not sure I follow Kauffman here. The toy world or the bucket brigades that Kadanoff and Anderson talk about are models. Models are very different from physical laws. Sure, there can be multiple models (or platforms) for deriving a given set of phenomena, but the existence of multiple models does not preclude dependence on a unique set of laws. A close analogy which I often think of is from molecular mechanics. A molecular mechanics model of a molecule assumes the molecule to be a classical set of balls and springs, with the electrons neglected. By any definition this is a ludicrously simple model that completely ignores quantum effects (or at least takes them into consideration implicitly by getting parameters from experiment). Yet, with the right parametrization, it works well-enough to be useful. There could conceivably be many other models which could give the same results. Yet nobody would make the argument that the behavior of molecules modeled in molecular mechanics is not reducible to quantum mechanics.

Kauffman’s argument that the explanatory arrows don’t always point downwards because one cannot always extrapolate upwards from lower-level phenomena is very well-taken. Emergent properties are surely real. But at least in the specific cases he considers, I am not sure that one can make an argument about phenomena not being reducible to specific physics simply because they can be derived from multiple platforms. The multiple platforms are models. The specific physics constitutes a set of laws, which is quite different.

The jewel of physics faces the 4% challenge

The size of the proton has shrunk by 4%, or so they tell us. The research which was published in Nature and has created waves apparently interrogated the proton with a much more reliable subatomic entity, the muon, which led to a more accurate result. The result of course testifies to the incredible power of modern science to divine such unbelievably small numbers.

But according to a NYT article, this might mean that the "jewel of physics", quantum electrodynamics, may be in trouble. QED which was developed by Richard Feynman and others is the most accurate theory known to science, and has calculated the magnetic moment of the proton to an accuracy of ten significant figures with respect to experiment. As Feynman himself said, this is like calculating the distance between New York and New Orleans to within the width of a hair.

The present measurement could shake up this success a bit according to the article:

When that new radius, which is 10 times more precise than previous values, was used to calculate the Rydberg constant, a venerable parameter in atomic theory, the answer was 4 percent away from the traditionally assumed value. This means there are now two contradicting values of the Rydberg constant, Dr. Pohl explained, which means there is either something wrong with the theory, quantum electrodynamics, or the experiment.

“They are completely stunned by this,” said Dr. Pohl of his colleagues. “They are working like mad. If there is a problem with quantum electrodynamics this will be an important step forward.”

The late Caltech physicist Richard Feynman called quantum electrodynamics “the jewel of physics,” and it has served as a template for other theories.

One possibility is that there is something physics doesn’t know yet about muons that throws off the calculations.

Or perhaps something we just don’t know about physics. In which case, Jeff Flowers of the National Physical Laboratory in Teddington in Britain pointed out in a commentary in Nature, a new phenomenon has been discovered not by the newest $10 billion collider but by a much older trick in the book, spectroscopy.

“So, if this experimental result holds up, it is an open door for a theorist to come up with the next theoretical leap and claim their Nobel Prize,” Dr. Flowers wrote.

In other news, a physicist has postulated that gravity is not really a fundamental force but could be a manifestation of the second law of thermodynamics.

Who said challenges do not abound in modern physics!

Computational modeling of GPCRs: What are the challenges?

GPCRs are extremely important proteins both for pure and applied science research, but they are also very difficult to crystallize and hence structural information on them has been sparse. Naturally in such a case, computational modeling can be expected to be of great value of providing insight into GPCR structure and function. However, even though progress has been impressive, such modeling still has to overcome many challenges. A recent review lists some of them.

Firstly, in the absence of crystal structure, homology modeling wherein a sequence for an unknown structure is 'threaded' through that of a known one is well-established as a valuable technique. However the technique is tricky. First and foremost one has to get the right sequence alignment between the target and the template. As the article notes, recent studies have suggested that using multiple structures for alignment instead of a single one provides better results. Particularly noteworthy is this detailed study. Once a homology model has been obtained, it must be meticulously examined, both for internal consistency (bad contacts, incorrect hydrogen bonding interactions etc.) and for its agreement with experiment. Data from cross-linking studies and mutagenesis can be used to achieve this. A recent promising development has been termed 'ligand-supported homology modeling'. In this process, topographical protein-ligand interaction data from mutagenesis and other studies is used to limit the number of homology models. Such data-driven homology modeling is becoming increasingly popular.

Once a good homology model has been obtained, many things can be done with it. Molecular dynamics (MD) simulations provide a very valuable avenue for exploring protein motion and be used to detect structural features not obvious in static models. A recent MD simulation of the beta-adrenergic receptor helped to resolve discrepancies between biochemical and structural observations. MD simulations can be used to investigate protein dynamics and to refine the models. Several challenges present themselves during this procedure. Firstly, while helices in GPCRs can be well-modeled, loops (of which there are six- three intracellular and three extracellular) are much harder to model because of their higher flexibility and because they are often ill-resolved in crystal structures. Unfortunately, it's these loops which are important ligand-interacting elements, so getting them right is key. Recently developed algorithms for loop-refinement based on either first-principles energy minimization or by statistical modeling based on a database of known loop conformations have been used in getting loops right. Also, state-of-the-art long MD simulations spanning several microseconds can be used to model large-scale structural changes in GPCRs.

There are still immense challenges still to be overcome in understanding GPCRs. One of the biggest concerns the cycling between several inactive and active states (and not just one active and one inactive state) that present often conflicting features that can be subject to varying interpretation. For instance, for class A GPCRs (which is the largest class), it has been well-established that activated states involve the breakage of the "ionic lock", a salt bridge between arginines and glutamates on transmembrane helices 6 and 3. Breaking this lock allows TM6 to shift away from TM3 and towards TM5, a hallmark of GPCR activation. Yet the MD study on the beta2 cited above indicated that even an inactive state may feature breakage of this lock.

In the GPCR jungle, strange shape-shifting creatures appear and clutch gems of insight in their palms. It is only fitting that we throw the kitchen sink at them to unravel their secrets, and computational techniques can only be a valuable arrow in this quiver.

Yarnitzky T, Levit A, & Niv MY (2010). Homology modeling of G-protein-coupled receptors with X-ray structures on the rise. Current opinion in drug discovery & development, 13 (3), 317-25 PMID: 20443165

Lindau 2010: Island Full of Ideas

I am very fortunate in being invited again to blog for the 60th Meeting of Nobel Laureates in Lindau, Germany. This year's interdisciplinary star-cast features more than 60 Nobel Prize winners from physics, chemistry and medicine and more than 600 young students and researchers from around the world. It's been a pleasure blogging for this one-of-a-kind meeting. Below are listed some of my posts with excerpts. You can click on the titles to read the full posts.

1. Reflections on Nobel City

Cities, just like human beings, have character. The character is frequently defined by little things as well as big. For instance New York is The Big Apple, Paris the city of fashion, Sydney the city with the Opera House and Rio de Janeiro the carnival city. Small cities are also known for their own accomplishments. For instance, last year I visited the the little German city of Magdeburg which is known for Otto von Guericke, the man who established the physics of vacuums through a famous experiment involving horses...

2. Microwaves, Magnetism and Machine Grease: A Paean to Tool-Driven Science

John Turton Randall was trying hard, real hard. For some time now, the University of Birmingham physicist was focusing on trying to improve the features of a machine which transmitted and received electromagnetic waves. A few years back this would have been just another intriguing academic problem for a physicist to crack, but this time it was a matter of life and death for thousands. Literally. It was 1939, and an ominous menace loomed large over Europe in the person of Adolf Hitler. The machine Randall was working on was designed to thwart Hitler's attempts to invade the British mainland. It sent out electromagnetic waves of meter wavelength and tried to deduce the position of an object based on its reflection of these waves. The operating principle of this humble machine later turned into a household name- Radar...

3. Pigeon Waste, Cosmic Melodies and Noise in Scientific Communication

There it was, that darned noise again.

Nobody could possibly be happy cleaning pigeon droppings. Yet Arno Penzias and Robert Wilson were being forced to do it. As good scientists they simply could not avoid it, since they had to discount the role of this "white dielectric substance" in the noise that was plaguing their equipment. When they finished with the cleaning and dispatched the pigeons by mail to a faraway place, the noise still did not disappear. And it seemed to come from all directions. The implications of this annoying constant background hum, corresponding to a temperature of only 3 degrees above absolute zero, signified one of the most momentous discoveries in twentieth-century physics, notable even among Nobel Prize-winning discoveries...

4. Paul Crutzen's Other Big Idea

Nobel Laureate Paul Crutzen will be at Lindau this year, along with his fellow recipient F. Sherwood Rowland. The two along with Mario Molina contributed to one of the most significant intersections of science with politics and public policy in the twentieth century when they discovered the effects of chlorofluorocarbons and other chemical compounds on the all-important ozone layer. Crutzen is well-known for that contribution...

5. Mountains Beyond Mountains

The scientist, by the very nature of his commitment, creates more and more questions, never fewer. Indeed, the measure of our intellectual maturity is our capacity to feel less and less satisfied with our answers to better problems.- G.W. Allport, Becoming, 1955

Science in the popular mind consists of a series of "Eureka!" moments. Such moments are supposed to suddenly propel scientific fields ahead at accelerating rates. Many anecdotes from scientific history seem to confirm this belief. It all begins with Archimedes jumping out of the bath after discovering the principle of buoyancy. Other examples include the apple falling on Isaac Newton’s head, August Kekule waking up from a dream and realizing the structure of benzene, Enrico Fermi discovering slow neutrons by ‘randomly’ substituting a block of paraffin for a tabletop, Alexander Fleming ‘accidentally’ discovering the action of a famous mold on bacteria, and Werner Heisenberg discovering the awesome structure of the quantum world after an all-night session on the island of Heligoland in the North Sea...

6. Heisenberg and Dirac

Beatrice's story about Heisenberg possibly inspiring the "Schunkelwalzer" dancing tradition at Lindau reminds me of an ancedote about Heisenberg and Paul Dirac. Both were two of the most accomplished scientists of the twentieth century who made foundational contributions to quantum mechanics. But while Heisenberg loved song, dance and wine, Dirac was a very quiet man and a singularly unusual character who generously extended his abstract thinking to interpreting the world literally. This inevitably led him to being an anecdote generator throughout his life and many stories about him abound. Here are a few, concluding with the story about him and Heisenberg...

7. Infections and Disease: The Golden Age

Harald zur Hausen's discovery of the link between infection and cancer provides a window into what may turn out to be one of the most fascinating lines of inquiry in twenty-first century medical research: the link between microorganisms and what have been traditionally considered chronic diseases.

This line of inquiry is founded on an evolutionary truth. Bacteria and viruses have been human beings' most constant companions, existing on this planet billions of years before we did and greeting us as we climbed out of the trees and walked out of Africa. Since the very beginning we have been engaged in an arms race with microbes. The conventional wisdom is that these arms races have led to an essentially benign co-existence between us and "them". But recent thinking has challenged this widespread belief and the truth appears to be more complicated...

Parallel worlds, parallel lives

"Parallel Worlds, Parallel Lives" is a documentary about Hugh Everett, the fascinating, brilliant and troubled physicist who conceived the idea of parallel universes, which have become a staple of science fiction ever since and are now being taken seriously even by serious scientists.

Everett received his PhD. from Princeton in the 1950s with John Wheeler. At the time the prevailing view of what happens when you observe a quantum system was the Copenhagen Interpretation which said that until you observe a quantum system it exists in a superposition of states; the wavefunction of such a system suddenly "collapses" when you observe it. This of course led to several dilemmas and paradoxes, the most famous one being Schrodinger's Cat. Several questions arose; when exactly does the wavefunction collapse? Who can collapse it? Everett bypassed the whole problem by assuming that quantum systems simply exist in many different states but in separate universes and you observe one of them. Thus the wavefunction does not collapse at all. This of course sounded fantastic, implying that at every moment, there is a copy of you for instance that splits into infinitely many copies in infinitely many universes. However, it did seem to provide a simple way out of Schrodinger's cat-type problems. The "many-worlds interpretation" of quantum mechanics has fascinated, troubled and interested scientists and laymen alike ever since.

Unfortunately, Bohr's "gospel" prevailed among physicists, and Bohr strongly disagreed with Everett in a meeting that Wheeler had set up between them. Disappointed and with a family history of depression, Everett left academia for good. He spent the rest of his life doing top-secret work for the government, coming up with algorithms and computer programs for modeling nuclear war. He apparently was very influential in suggesting nuclear weapons policy which the government adopted and several of his reports are still highly classified. One of the concepts he pioneered was the Lagrange multipliers method, a key tool in solving differential equations with constraints in diverse disciplines. He died suddenly of a heart attack in the 1970s. Everett had a drinking problem and a tragic family life. He was very distant from his children. His son who is the main subject in the documentary says that the only time he touched his father physically was after he died and the dead body had to be moved. Everett's daughter committed suicide, writing a bizarre suicide note saying that she was going to meet her father in a parallel universe.

The documentary is a NOVA documentary on PBS. It's about Everett's son, the musician Mark Everett (who seems to be quite successful with his band "The Eels") who sets out on a journey to Princeton, the Pentagon, Austin, Cambridge etc. to find out more about his father and speaks to such people as Charles Misner and David Deutsch. On the way he learns some quantum mechanics and gets to know his father much better. In the end he feels much closer to his father and seems to have finally received closure. It was rather touching to be honest and there is a sense of satisfaction in his son finally seeming to be at peace.

Note: A rather expensive biography of Everett has just come out. A cheaper, free version is a short Scientific American piece on him.

The origin of life cannot escape basic organic chemistry

One of the key challenges facing any theories of the molecular origins of life concerns the synthesis, stability polymerization and self-assembly of early life's molecular components. If you cannot explain the chemical origin of these components, you cannot really explain the origin of life. In case of life as we know it, this boils down to explaining the origin of the building blocks of living organisms, namely nucleotides and amino acids.

The simplest principles and quirks of chemistry could have had an influence on how life could have evolved. A neat paper in ACS Chemical Biology offers a potential explanation based on basic organic chemistry for why a certain class of phosphorylated nucleotides formed in preference to others, even though 'conventional' organic chemistry would dictate the opposite.

An anhydroarabinonucleoside has been postulated as an important potential precursor to further nucleotide synthesis. A key step is the phosphorylation of this nucleoside to yield an activated cyclic nucleoside phosphate. Having an activated molecule makes all the difference since activation primes the molecule to be attacked by further nucleophiles, thus triggering polymerization and growth.

However, the phosphorylation of the arabinose nucleoside raises a fundamental question (hopefully) familiar to sophomore organic chemistry students. Why does phosphorylation take place preferentially on the secondary 3'-OH while sterically, as every student of organic chemistry knows, it should be preferred much more on the primary 5'-OH?

To tackle this question, the authors get a crystal structure of the nucleoside in question. This x-ray structure shows an unusually short distance between the 2'-OH oxygen and the C2 carbon (2.7 A, a).


Energy optimization using quantum chemical techniques surprisingly does not get rid of the short distance. Because of this proximity, the 2'-OH can undergo an internal attack on this carbon to generate a reactive intermediate (1), whose ring can be opened in turn by a 3'-OH phosphate to form the activated phosphate product. Now, the 5'-OH also gets phosphorylated; it's just that it cannot attack the C3 carbon of the activated intermediate the way the 2'-OH can because it's not in proximity to this carbon the way the 2'-OH is.


The authors explain the short distance between the 2'-OH and the C3 carbon by postulating an interaction between the lone pair of the 2'-OH oxygen and the pi* orbital of the C2=N bond. This kind of interaction is quite familiar to organic chemists; it is invoked in the famous Burgi-Dunitz trajectory that enables nucleophilic attack on carbonyl carbons. Indeed, the authors perform a theoretical analysis that shows the angle of attack for the 2'-OH to be about a 100 degrees, close enough to the Burgi-Dunitz trajectory.

This is a classic case of there being two competing pathways in chemistry, one of which is preferred to the other because of a subsequent low-energy route that can be traversed. It's a common theme in chemistry and biochemistry and illustrates how otherwise counter-intuitive reactions can be accelerated by putting them at the top of the right energy cliffs. No matter how complex life may be, it still cannot get around the basic laws of organic chemistry. Score one for thermodynamics.

Choudhary, A., Kamer, K., Powner, M., Sutherland, J., & Raines, R. (2010). A Stereoelectronic Effect in Prebiotic Nucleotide Synthesis ACS Chemical Biology DOI: 10.1021/cb100093g

The aesthete

I had a great time visiting Santa Fe and Los Alamos over the weekend. At Los Alamos there is a nice little museum in Fuller Lodge, where the Manhattan Project scientists used to socialize on weekends. One of the amusing artifacts there is a set of two letters sent by Oppenheimer's secretary asking for a nail to be driven into the wall so that he could hang his hat. There is the first letter...and then there is the follow up.


It's remarkable that this intellectual aesthete did not have the practical drive to hammer a nail into the wall. One could not have imagined someone like Fermi or Feynman leaving the problem unattended for so long. In light of this it seems even more astonishing that a dyed-in-the-wool hands-off theoretician like Oppenheimer could not only direct a world-class group of Nobel Prize winning scientists and engineers to achieve the impossible in record time, but also keep the most practical details of an unimaginably vast project in his head. He even knew who was the best person in the country to manage the organic chemistry stockroom.

Physicist Victor Weisskopf of MIT said it well:

"He did not direct from the head office. He was intellectually and even physically present at each decisive step. He was present in the laboratory or in the seminar rooms, when a new effect was measured, when a new idea was conceived. It was not that he contributed so many ideas or suggestions; he did so sometimes, but his main influence came from something else. It was his continuous and intense presence, which produced a sense of direct participation in all of us; it created that unique atmosphere of enthusiasm and challenge that pervaded the place throughout its time"

Conformations of the stevastelins: A reassessment

Shameless self-promotion: my paper on the conformational analysis of cyclic antiviral peptides called stevastelins is now online on the Biopolymers site. Here's a brief overview.

The stevastelins are cyclic peptides that show promising antiviral activity against the vaccinia viral VHR phosphatase. These peptides are phosphorylated in vivo before they can inhibit their target protein. A group in Germany previously did a meticulous analysis of four diastereometric analogs of these peptides which included their synthesis, biological characterization and conformational analysis. However, the conformational analysis was done using force field conformational searches from a single force field, constrained by variables from the NMR data (coupling constant derived dihedral angles and NOESY derived distances). Using such a protocol, the group concluded that each of the four diastereomers exists as a single conformational family in solution.

The problem with constrained conformational searches (or constrained molecular dynamics for that matter) is that they constitute a rather self-fulfilling exercise, with the assumption that there is in fact a single conformation of the molecule under question. However, as I have often discussed on this blog, any molecule with a couple of rotatable bonds is going to exist as multiple conformers in solution, so an assumption of a single conformation would be fuzzy unless supported by more data. NMR by itself is of scant value in determining these conformations for thermodynamic and kinetic reasons. Plus, analyzing conformations using a single force field can be fraught with ambiguity, since every force field comes with its own set of parameters and convergence criteria. Especially trusting energies from force fields can be dangerous. In this case, the stevastelin peptides have 9 rotatable bonds each, so I thought it worthwhile to apply our previously developed and applied NAMFIS (NMR Analysis of Molecular Flexibility In Solution) methodology combining NMR variables with structures from extensive conformational searches to the enumeration of the conformational behavior of these interesting molecules.

The paper essentially describes the conformational variability obtained for each of the diastereomers. Many of the conformations are very similar to the previously postulated families, but some are quite different. There are also some striking observations that are corroborated; for instance, the use of a d-serine truly seems to 'lock' the peptides in a single conformation. Such a lock could be effected to counter the entropic penalty that a multiconformational ensemble of molecules might have to pay. The instructive general observation is that subtle changes in sterechemistry at one or two chiral centers can dramatically affect conformational behavior, a fact that continues to surprise and confound medicinal chemists. I also note that if the NMR data for the phosphorylated peptides were available, an interesting comparison of the conformational pool for the phosphorylated and unphosphorylated counterparts could be attempted. This would shed light on whether phosphorylation leads to less conformational variability or simply increases the proportion of a chosen subset of conformations of the peptides.

Comments, criticism and general feelings of chagrin are welcomed.

Jogalekar, A. (2010). Conformations of stevastelin C3 analogs: Computational deconvolution of NMR data reveals conformational heterogeneity and novel motifs Biopolymers DOI: 10.1002/bip.21504

A shot in the arm for antimalarial drug discovery?

While heart disease, cancer and Alzheimer's continue to grab the headlines, malaria and tuberculosis continue to quietly do their deadly work behind the scenes. Diseases that disproportionately affect sub-Saharan Africa are not exactly priorities for drug companies. But they pose a tremendous unmet need. Especially malaria, which kills an unbelievable 800,000 people every year, has fought back against almost every traditional drug. The fight against the disease has boiled down to one class of drugs- the artemisinins. If the parasite develops resistance against these, nobody knows how fast and wide it will spread.

Since pharma companies often get bad press for neglecting....neglected diseases, this makes the duo of papers in this week's issue of Nature especially impressive. The papers talk about GSK collaborating with a host of academic laboratories to discover literally hundreds of hits against malaria through phenotypic screening. The sheer multidisciplinary effort put into this endeavor is laudable. Phenotypic screening is an effective method for drug discovery since it does not care about the target of a drug, at least in the beginning. It's a more top down approach that complements bottom-up rational drug design. The goal is to simply watch out for a particular kind of response, which could be anything from fluorescence to cell shrinkage. In this case it was 80% inhibition of growth of the parasite in the asexual stage in red blood cells. Target identification can come later.

The company screened its proprietary collection of about 2 million compounds. The compound library was chosen for diversity of scaffolds and novel chemotypes. The assay looked for 80% inhibition of the P. falciparum parasite, and came up with hundreds of diverse compounds. The scientists seemed to have taken due care to minimize false positives. They sought to eliminate promiscuous, lipophilic compounds from the list. They also screened their compounds against well known targets and processes that the malarial parasite exploits to subdue its host. One of these was particularly eye-opening for me; apparently, the insidious little weasel can hack up the amino acids from hemoglobin molecules in the host to assemble its own proteins. Now that's stealth for you. More interestingly, the group then screened the selected molecules against seven novel malarial targets and found encouraging inhibition profiles against these targets. Infectious disease are best treated when you can hit the causative agent in multiple places. Paucity of targets has especially been an issue for malaria and TB, and these chemotypes along with their suggested targets provide promising leads. As a final act, the first paper co-authored by Guiguemde et al. also demonstrates favorable pharmacokinetic properties for one of their hits.

Especially interesting is the report in the second paper authored by Gamo et al. where the authors follow a similar procedure but discover that the novel target list for the purported antimalarial candidates is enriched in kinases. They take due care to investigate that this enrichment is not a chance enrichment. Unlike the human genome which has about 500 kinases, the malarial genome has about 80. But finding kinases among the targets of these novel chemotypes has rich implications, since kinases have already been intensely investigated, the targets are well-understood and there are literally thousands of kinase inhibitors out there waiting to be tested. Testing kinase inhibitors against malaria would open up a whole new chapter for antimalarial drug discovery.

Finally, and this is the kicker most talked about, GSK has made the entire list of hits freely available to the public. This is a very laudable act. In an age where corporations are routinely derided for their emphasis on secrecy and profit-making, such a decision should drive home the good work that corporations can potentially do. It also underscores the tremendous opportunities for drug discovery against neglected diseases gained from academic-corporate collaboration. While it remains to be seen how many of these promising candidates become bona fide drugs, it provides many promising starting points for further efforts. Malaria is about as insidious a disease as you can have, lurking in the shadows and waiting to pounce on you. The more the hands that try to squeeze its neck, the better.

Guiguemde, W., Shelat, A., Bouck, D., Duffy, S., Crowther, G., Davis, P., Smithson, D., Connelly, M., Clark, J., Zhu, F., Jiménez-Díaz, M., Martinez, M., Wilson, E., Tripathi, A., Gut, J., Sharlow, E., Bathurst, I., Mazouni, F., Fowble, J., Forquer, I., McGinley, P., Castro, S., Angulo-Barturen, I., Ferrer, S., Rosenthal, P., DeRisi, J., Sullivan, D., Lazo, J., Roos, D., Riscoe, M., Phillips, M., Rathod, P., Van Voorhis, W., Avery, V., & Guy, R. (2010). Chemical genetics of Plasmodium falciparum Nature, 465 (7296), 311-315 DOI: 10.1038/nature09099

Gamo, F., Sanz, L., Vidal, J., de Cozar, C., Alvarez, E., Lavandera, J., Vanderwall, D., Green, D., Kumar, V., Hasan, S., Brown, J., Peishoff, C., Cardon, L., & Garcia-Bustos, J. (2010). Thousands of chemical starting points for antimalarial lead identification Nature, 465 (7296), 305-310 DOI: 10.1038/nature09107

Life anew?

The recent creation of a "synthetic organism" by Craig Venter and his colleagues his hit the headlines. By all accounts it is a thoroughly impressive piece of work, a tour de force that designed a genome from scratch, literally by writing it the way a piece of computer code is written. The perseverance and ingenuity put into the process deserve ample applause. And it should rightly catapult the emerging field of synthetic biology into the public discourse.

But it's still not a "synthetic cell" in my opinion. The genome was inserted into a cell where it started working exactly as expected. I would not hold my breath before we can design completely synthetic genomes that can do whatever we want, including eating CO2 or producing Lipitor.

To this non-expert, the reason looks simple: everything in molecular biology that we have encountered until now has turned out to be more complex than expected. Notice what's happened to AIDS vaccines, gene therapy and treatments for Alzheimer's disease, all of which would supposedly be simpler than designing a synthetic organism. In each of these cases, what seemed obvious and straightforward has turned out to be a maze of unexpected challenges and unexplained observations. The fact is, designing a genome is one thing, making it produce proteins that will interact with each other in a carefully orchestrated manner, will find binding partners with exquisite specificity and accomplish the extremely complex and often non-intuitive cascades of signal transduction is quite another. A cell is not just the genome, it's really about interactions. And I am willing to bet that we are a long way off before we can generally design all those countless specific interactions which give rise to that entity named a "cell".